› 1cjg

Solution NMR

NMR STRUCTURE OF LAC REPRESSOR HP62-DNA COMPLEX

Released:
DOI: 10.2210/pdb1cjg/pdb
PDB entry 1cjg coloured by chain, ensemble of 11 models, front view.
PDB entry 1cjg coloured by chain, ensemble of 11 models, side view.
PDB entry 1cjg coloured by chain, ensemble of 11 models, top view.
Source organism: Escherichia coli
Primary publication:
The solution structure of Lac repressor headpiece 62 complexed to a symmetrical lac operator.
Spronk CA, Bonvin AM, Radha PK, Melacini G, Boelens R, Kaptein R
Structure 7 1483-92 (1999)
PMID: 10647179

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113543 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Lactose operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 62 amino acids
Theoretical weight: 6.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-62; Coverage: 17%)
Gene names: JW0336, b0345, lacI
Sequence domains: Bacterial regulatory proteins, lacI family
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*TP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 6.75 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: SIMULATED ANNEALING FOLLOWED BY RESTRAINED MD
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

The Lac repressor: a second generation of structural and functional studies.
Bell et al. (2001)
1 more

8 mentions without citation

An all-atom knowledge-based energy function for protein-DNA threading, docking decoy discrimination, and prediction of transcription-factor binding profiles.
Xu et al. (2009)
7 more