› 1cgp

X-ray diffraction
3Å resolution

CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

Released:
DOI: 10.2210/pdb1cgp/pdb
PDB entry 1cgp coloured by chain, front view.
PDB entry 1cgp coloured by chain, side view.
PDB entry 1cgp coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees.
Schultz SC, Shields GC, Steitz TA
Science 253 1001-7 (1991)
PMID: 1653449

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113541 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding transcriptional dual regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 205 amino acids
Theoretical weight: 23.06 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 2-206; Coverage: 98%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:
DNA (5'-D(*GP*CP*GP*AP*AP*AP*AP*GP*TP*GP*TP*GP*AP*CP*AP*TP*AP*T)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 18 nucleotides
Theoretical weight: 5.59 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*TP*CP*AP*CP*AP*CP*TP*TP*TP*TP*CP*G)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 13 nucleotides
Theoretical weight: 3.92 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CMP 2 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2221
Unit cell:
a: 138Å b: 152.6Å c: 76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 not available
Expression system: Not provided

Quick links

Citations

111 review citations

Transcription activation by catabolite activator protein (CAP).
Busby et al. (1999)
110 more

39 mentions without citation

An introduction to NMR-based approaches for measuring protein dynamics.
Kleckner et al. (2011)
38 more