› 1cf8

X-ray diffraction
2.7Å resolution

Convergence of catalytic antibody and terpene cyclase mechanisms: polyene cyclization directed by carbocation-pi interactions

Released:
DOI: 10.2210/pdb1cf8/pdb
PDB entry 1cf8 coloured by chain, front view.
PDB entry 1cf8 coloured by chain, side view.
PDB entry 1cf8 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Convergence of Catalytic Antibody and Terpene Cyclase Mechanisms: Polyene Cyclization Directed by Carbocation-pi Interactions
Paschall CM, Hasserodt J, Jones T, Lerner RA, Janda KD, Christianson DW
Angew. Chem. Int. Ed. Engl. 38 1743-1747 (1999)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-134669 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Immunoglobulin kappa constant Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.48 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-107; Coverage: 100%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Immunoglobulin heavy constant gamma 2B Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.5 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01867 (Residues: 1-98; Coverage: 24%)
Gene names: Igh-3, Ighg2b
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand CD 2 x CD
Ligand HAZ 1 x HAZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 101.3Å b: 70.3Å c: 69.6Å
α: 90° β: 114.2° γ: 90°
R-values:
R R work R free
0.165 0.165 0.257

Quick links

Citations

1 mention without citation

An anti-hapten camelid antibody reveals a cryptic binding site with significant energetic contributions from a nonhypervariable loop.
Fanning et al. (2011)