› 1c9p

X-ray diffraction
2.8Å resolution

COMPLEX OF BDELLASTASIN WITH PORCINE TRYPSIN

Released:
DOI: 10.2210/pdb1c9p/pdb
PDB entry 1c9p coloured by chain, front view.
PDB entry 1c9p coloured by chain, side view.
PDB entry 1c9p coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the complex of the antistasin-type inhibitor bdellastasin with trypsin and modelling of the bdellastasin-microplasmin system.
Rester U, Bode W, Moser M, Parry MA, Huber R, Auerswald E
J Mol Biol 293 93-106 (1999)
PMID: 10512718

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133389 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Trypsin Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.49 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P00761 (Residues: 9-231; Coverage: 97%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Bdellastasin Chain: B
Molecule details ›
Chain: B
Length: 59 amino acids
Theoretical weight: 6.35 KDa
Source organism: Hirudo medicinalis
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P82107 (Residues: 1-59; Coverage: 100%)
Sequence domains: Antistasin family
Structure domains: Antistasin; domain 1

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
1 modified residue:
Ligand IAS 1 x IAS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 63.33Å b: 63.33Å c: 130.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.185 0.227
Expression system: Saccharomyces cerevisiae

Quick links

Citations

5 review citations

Evolutionary families of peptidase inhibitors.
Rawlings et al. (2004)
4 more

1 mention without citation

Draft genome of the European medicinal leech Hirudo medicinalis (Annelida, Clitellata, Hirudiniformes) with emphasis on anticoagulants.
Kvist et al. (2020)