› 1bhu

Solution NMR

THE 3D STRUCTURE OF THE STREPTOMYCES METALLOPROTEINASE INHIBITOR, SMPI, ISOLATED FROM STREPTOMYCES NIGRESCENS TK-23, NMR, MINIMIZED AVERAGE STRUCTURE

Released:
DOI: 10.2210/pdb1bhu/pdb
PDB entry 1bhu coloured by chain, front view.
PDB entry 1bhu coloured by chain, side view.
PDB entry 1bhu coloured by chain, top view.
Source organism: Streptomyces nigrescens
Primary publication:
NMR structure of the Streptomyces metalloproteinase inhibitor, SMPI, isolated from Streptomyces nigrescens TK-23: another example of an ancestral beta gamma-crystallin precursor structure.
Ohno A, Tate S, Seeram SS, Hiraga K, Swindells MB, Oda K, Kainosho M
J Mol Biol 282 421-33 (1998)
PMID: 9735297

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133931 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metalloproteinase inhibitor Chain: A
Molecule details ›
Chain: A
Length: 102 amino acids
Theoretical weight: 10.37 KDa
Source organism: Streptomyces nigrescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01077 (Residues: 30-131; Coverage: 100%)
Gene name: smpI
Sequence domains: Peptidase inhibitor family I36
Structure domains: Gamma-B Crystallin; domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Ageing and vision: structure, stability and function of lens crystallins.
Bloemendal et al. (2004)
6 more

3 mentions without citation

Prokaryote-derived protein inhibitors of peptidases: A sketchy occurrence and mostly unknown function.
Kantyka et al. (2010)
2 more