1be0

X-ray diffraction
1.96Å resolution

HALOALKANE DEHALOGENASE AT PH 5.0 CONTAINING ACETIC ACID

Released:

Function and Biology Details

Reaction catalysed:
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-149750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 35.16 KDa
Source organism: Xanthobacter autotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P22643 (Residues: 1-310; Coverage: 100%)
Gene name: dhlA
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-21
Spacegroup: P21212
Unit cell:
a: 92.61Å b: 72.51Å c: 41.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.22
Expression system: Escherichia coli