› 1bcc

X-ray diffraction
3.16Å resolution

CYTOCHROME BC1 COMPLEX FROM CHICKEN

Released:
DOI: 10.2210/pdb1bcc/pdb
PDB entry 1bcc coloured by chain, front view.
PDB entry 1bcc coloured by chain, side view.
PDB entry 1bcc coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Electron transfer by domain movement in cytochrome bc1.
Zhang Z, Huang L, Shulmeister VM, Chi YI, Kim KK, Hung LW, Crofts AR, Berry EA, Kim SH
Nature 392 677-84 (1998)
PMID: 9565029

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-132267 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Cytochrome b-c1 complex subunit 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 446 amino acids
Theoretical weight: 49.8 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P31800 (Residues: 35-480; Coverage: 93%)
Gene name: UQCRC1
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Cytochrome b-c1 complex subunit 2, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 422 amino acids
Theoretical weight: 45.08 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P23004 (Residues: 32-453; Coverage: 93%)
Gene name: UQCRC2
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
Cytochrome b Chain: C
Molecule details ›
Chain: C
Length: 380 amino acids
Theoretical weight: 42.62 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P18946 (Residues: 1-380; Coverage: 100%)
Gene names: COB, CYTB, MT-CYB, MTCYB
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome c1, heme protein, mitochondrial Chain: D
Molecule details ›
Chain: D
Length: 241 amino acids
Theoretical weight: 27.36 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00125 (Residues: 85-325; Coverage: 74%)
Gene name: CYC1
Sequence domains: Cytochrome C1 family
Structure domains:
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chain: E
Molecule details ›
Chain: E
Length: 196 amino acids
Theoretical weight: 21.74 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P13272 (Residues: 79-274; Coverage: 72%)
Gene name: UQCRFS1
Sequence domains:
Structure domains:
Cytochrome b-c1 complex subunit 7 Chain: F
Molecule details ›
Chain: F
Length: 109 amino acids
Theoretical weight: 13.4 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00129 (Residues: 2-110; Coverage: 98%)
Gene name: UQCRB
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Structure domains: Cytochrome b-c1 complex subunit 7
Cytochrome b-c1 complex subunit 8 Chain: G
Molecule details ›
Chain: G
Length: 81 amino acids
Theoretical weight: 9.72 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P13271 (Residues: 2-82; Coverage: 99%)
Gene name: UQCRQ
Sequence domains: UcrQ family
Structure domains: Cytochrome b-c1 complex subunit 8
Cytochrome b-c1 complex subunit 6, mitochondrial Chain: H
Molecule details ›
Chain: H
Length: 78 amino acids
Theoretical weight: 9.22 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00126 (Residues: 14-91; Coverage: 86%)
Gene name: UQCRH
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Structure domains: Ubiquinol-cytochrome C reductase hinge domain
UBIQUINOL CYTOCHROME C OXIDOREDUCTASE Chain: I
Molecule details ›
Chain: I
Length: 33 amino acids
Theoretical weight: 2.83 KDa
Source organism: Gallus gallus
Cytochrome b-c1 complex subunit 9 Chain: J
Molecule details ›
Chain: J
Length: 62 amino acids
Theoretical weight: 7.18 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00130 (Residues: 2-63; Coverage: 97%)
Gene name: UQCR10
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like
Structure domains: Cytochrome b-c1 complex subunit 9

Ligands and Environments


Cofactor: Ligand HEM 3 x HEM
4 bound ligands:
Ligand U10 1 x U10
Ligand PEE 2 x PEE
Ligand BOG 1 x BOG
Ligand FES 1 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 169.59Å b: 182.518Å c: 240.573Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.27 0.27 0.31

Quick links

Citations

118 review citations

Membrane protein folding and stability: physical principles.
White et al. (1999)
117 more

30 mentions without citation

Structure of the avian mitochondrial cytochrome bc1 complex.
Berry et al. (1999)
29 more