› 1b8h

X-ray diffraction
3Å resolution

SLIDING CLAMP, DNA POLYMERASE

Released:
DOI: 10.2210/pdb1b8h/pdb
PDB entry 1b8h coloured by chain, front view.
PDB entry 1b8h coloured by chain, side view.
PDB entry 1b8h coloured by chain, top view.
Source organism: Escherichia phage RB69
Primary publication:
Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex.
Shamoo Y, Steitz TA
Cell 99 155-66 (1999)
PMID: 10535734

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131314 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sliding clamp Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 228 amino acids
Theoretical weight: 25.13 KDa
Source organism: Escherichia phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: O80164 (Residues: 1-228; Coverage: 100%)
Gene name: 45
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
DNA-directed DNA polymerase Chain: D
Molecule details ›
Chain: D
Length: 11 amino acids
Theoretical weight: 1.35 KDa
Source organism: Escherichia phage RB69
Expression system: Not provided
UniProt:
  • Canonical: Q38087 (Residues: 893-903; Coverage: 1%)
Gene name: 43

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 67Å b: 90.5Å c: 146Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.265 0.313
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

48 review citations

Bacteriophage T4 genome.
Miller et al. (2003)
47 more

4 mentions without citation

Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives.
Mueser et al. (2010)
3 more