› 1b77

X-ray diffraction
2.1Å resolution

BUILDING A REPLISOME STRUCTURE FROM INTERACTING PIECES: A SLIDING CLAMP COMPLEXED WITH AN INTERACTION PEPTIDE FROM DNA POLYMERASE

Released:
DOI: 10.2210/pdb1b77/pdb
PDB entry 1b77 coloured by chain, front view.
PDB entry 1b77 coloured by chain, side view.
PDB entry 1b77 coloured by chain, top view.
Source organism: Escherichia phage RB69
Primary publication:
Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex.
Shamoo Y, Steitz TA
Cell 99 155-66 (1999)
PMID: 10535734

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sliding clamp Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 228 amino acids
Theoretical weight: 25.13 KDa
Source organism: Escherichia phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: O80164 (Residues: 1-228; Coverage: 100%)
Gene name: 45
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 65.9Å b: 87.6Å c: 138.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.269
Expression system: Escherichia coli

Quick links

Citations

48 review citations

Bacteriophage T4 genome.
Miller et al. (2003)
47 more

6 mentions without citation

Loading clamps for DNA replication and repair.
Bloom LB. (2009)
5 more