› 1b5i

X-ray diffraction
1.9Å resolution

OLIGO-PEPTIDE BINDING PROTEIN (OPPA) COMPLEXED WITH KNK

Released:
DOI: 10.2210/pdb1b5i/pdb
PDB entry 1b5i coloured by chain, front view.
PDB entry 1b5i coloured by chain, side view.
PDB entry 1b5i coloured by chain, top view.
Source organisms:
Primary publication:
Crystallographic and calorimetric analysis of peptide binding to OppA protein.
Sleigh SH, Seavers PR, Wilkinson AJ, Ladbury JE, Tame JR
J Mol Biol 291 393-415 (1999)
PMID: 10438628

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138914 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Periplasmic oligopeptide-binding protein OppA Chain: A
Molecule details ›
Chain: A
Length: 517 amino acids
Theoretical weight: 58.88 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: P06202 (Residues: 27-543; Coverage: 100%)
Gene names: STM1746, oppA
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle
Structure domains:
PROTEIN (LYS-ASN-LYS) Chain: B
Molecule details ›
Chain: B
Length: 3 amino acids
Theoretical weight: 390 Da
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand U1 8 x U1
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 109.689Å b: 76.089Å c: 70.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.223
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

Water mediation in protein folding and molecular recognition.
Levy et al. (2006)
12 more

9 mentions without citation

Rapid and accurate prediction and scoring of water molecules in protein binding sites.
Ross et al. (2012)
8 more