› 1b2z

X-ray diffraction
2.03Å resolution

DELETION OF A BURIED SALT BRIDGE IN BARNASE

Released:
DOI: 10.2210/pdb1b2z/pdb
PDB entry 1b2z coloured by chain, front view.
PDB entry 1b2z coloured by chain, side view.
PDB entry 1b2z coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
A structural double-mutant cycle: estimating the strength of a buried salt bridge in barnase.
Vaughan CK, Harryson P, Buckle AM, Fersht AR
Acta Crystallogr D Biol Crystallogr 58 591-600 (2002)
PMID: 11914482

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 110 amino acids
Theoretical weight: 12.4 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00648 (Residues: 48-157; Coverage: 89%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-13
Spacegroup: P32
Unit cell:
a: 57.75Å b: 57.75Å c: 81.28Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.17 0.228
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Recent advances in Cys-loop receptor structure and function.
Sine et al. (2006)
2 more