› 1au7

X-ray diffraction
2.3Å resolution

PIT-1 MUTANT/DNA COMPLEX

Released:
DOI: 10.2210/pdb1au7/pdb
PDB entry 1au7 coloured by chain, front view.
PDB entry 1au7 coloured by chain, side view.
PDB entry 1au7 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility.
Jacobson EM, Li P, Leon-del-Rio A, Rosenfeld MG, Aggarwal AK
Genes Dev 11 198-212 (1997)
PMID: 9009203

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113440 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Pituitary-specific positive transcription factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 17 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P10037 (Residues: 128-273; Coverage: 50%)
Gene names: Ghf-1, Pit-1, Pit1, Pou1f1
Sequence domains:
Structure domains:
CONSENSUS DNA 25-MER Chain: C
Molecule details ›
Chain: C
Length: 25 nucleotides
Theoretical weight: 7.72 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
DNA (5'-D(*CP*TP*TP*CP*CP*TP*CP*AP*TP*GP*TP*AP*TP*AP*TP*AP*C P*AP*TP*GP*AP*GP* GP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.34 KDa
Source organism: Rattus norvegicus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P1
Unit cell:
a: 42.5Å b: 50.1Å c: 55.8Å
α: 76.7° β: 79.3° γ: 67.2°
R-values:
R R work R free
0.23 0.23 0.302
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

21 review citations

The origin of protein interactions and allostery in colocalization.
Kuriyan et al. (2007)
20 more

16 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
15 more