› 1ab4

X-ray diffraction
2.8Å resolution

59KDA FRAGMENT OF GYRASE A FROM E. COLI

Released:
DOI: 10.2210/pdb1ab4/pdb
PDB entry 1ab4 coloured by chain, front view.
PDB entry 1ab4 coloured by chain, side view.
PDB entry 1ab4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the breakage-reunion domain of DNA gyrase.
Morais Cabral JH, Jackson AP, Smith CV, Shikotra N, Maxwell A, Liddington RC
Nature 388 903-6 (1997)
PMID: 9278055

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit A Chain: A
Molecule details ›
Chain: A
Length: 493 amino acids
Theoretical weight: 55.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AES4 (Residues: 30-522; Coverage: 56%)
Gene names: JW2225, b2231, gyrA, hisW, nalA, parD
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: I41
Unit cell:
a: 119.63Å b: 119.63Å c: 94.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.31
Expression system: Escherichia coli

Quick links

Citations

57 review citations

DNA topoisomerases: structure, function, and mechanism.
Champoux JJ. (2001)
56 more

30 mentions without citation

Topoisomerases as anticancer targets.
Delgado et al. (2018)
29 more