› 1a0b

X-ray diffraction
2.06Å resolution

HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1a0b/pdb
PDB entry 1a0b coloured by chain, front view.
PDB entry 1a0b coloured by chain, side view.
PDB entry 1a0b coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Insights into multistep phosphorelay from the crystal structure of the C-terminal HPt domain of ArcB.
Kato M, Mizuno T, Shimizu T, Hakoshima T
Cell 88 717-23 (1997)
PMID: 9054511

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142431 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aerobic respiration control sensor protein ArcB Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 14.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEC3 (Residues: 654-778; Coverage: 16%)
Gene names: JW5536, arcB, b3210
Sequence domains: Hpt domain
Structure domains: HPT domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 30.56Å b: 34.93Å c: 110.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.271
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Two-component signal transduction.
Stock et al. (2000)
25 more

7 mentions without citation

Diversity of structure and function of response regulator output domains.
Galperin MY. (2010)
6 more