› 117e

X-ray diffraction
2.15Å resolution

THE R78K AND D117E ACTIVE SITE VARIANTS OF SACCHAROMYCES CEREVISIAE SOLUBLE INORGANIC PYROPHOSPHATASE: STRUCTURAL STUDIES AND MECHANISTIC IMPLICATIONS

Released:
DOI: 10.2210/pdb117e/pdb
PDB entry 117e coloured by chain, front view.
PDB entry 117e coloured by chain, side view.
PDB entry 117e coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The R78K and D117E active-site variants of Saccharomyces cerevisiae soluble inorganic pyrophosphatase: structural studies and mechanistic implications.
Tuominen V, Heikinheimo P, Kajander T, Torkkel T, Hyytiä T, Käpylä J, Lahti R, Cooperman BS, Goldman A
J Mol Biol 284 1565-80 (1998)
PMID: 9878371

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.24 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00817 (Residues: 2-287; Coverage: 100%)
Gene names: IPP1, PPA, PPA1, YBR011C, YBR0202
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

2 bound ligands:
Ligand MN 8 x MN
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 58.4Å b: 102.8Å c: 116.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.24
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Toward a quantum-mechanical description of metal-assisted phosphoryl transfer in pyrophosphatase.
Heikinheimo et al. (2001)
9 more

5 mentions without citation

Transcriptional Profiling Identifies Location-Specific and Breed-Specific Differentially Expressed Genes in Embryonic Myogenesis in Anas Platyrhynchos.
Zhang et al. (2015)
4 more