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Title:4.0 Angstrom two-fold imposed cryo-EM map of TRiC in the both-ring closed ATP-AlFx state
Authors:Y.Cong,M.L.Baker,J.Jakana,D.Woolford,E.J.Miller,S.Reissmann,R.N.Kumar,A.M.Redding-Johanson,T.S.Batth,A.Mukhopadhyay,S.J.Ludtke,J.Frydman,W.Chiu
Sample:bovine TRiC (also called CCT)
Aggregation state:Single particle (4.0 angstroms resolution)
Red flagLatest update:2011-07-08
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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Sample
Sample name: bovine TRiC (also called CCT)
Theoretical molecular weight of the sample: 1.0
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinTRiC or CCT16-merfalseBos taurus
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL200-mesh Quantifoil holey grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE95%101 KNONEtwo-side blotting for 1 second before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 3200FSC300 kVFLOOD BEAMBRIGHT FIELD4.1 mm1000 nm3000 nm50000FIELD EMISSION GUNKodak SO163 film mmobjective lens astigmatism correction

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side entryJEOL 3200FSC CRYOHOLDER0°0°in-column omega energy filter0-20 eV101 K101 K K mrad18 e/Å201-AUG-2008
Processing
Software:EMAN
CTF correction:each micrograph
Resolution by author:4.0 Å
Resolution method:FSC at 0.5 cut-off
Processing details:A recently developed 2-D fast rotational matching (FRM2D) algorithm for image alignment, available in EMAN 1.8, was adopted in the refinement steps
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
15006.35 μm/pixellinkNIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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