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Title:TBP-containing S. pombe TFIID
Authors:H.Elmlund,V.Baraznenok,T.Linder,Z.Szilagyi,R.Rofougaran,A.Hofer,H.Hebert,M.Lindahl,C.M.Gustafsson
Sample:Native S. pombe TFIID
Aggregation state:Single particle (8 angstroms resolution)
Red flagLatest update:2011-01-10
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Sample
Sample name: Native S. pombe TFIID
Theoretical molecular weight of the sample: 0.87
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinTAF1-TAF12, TBPfalseS. pombeGO:0005669IPR003228
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 2010F120 kVOTHERBRIGHT FIELD mm nm nmFIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entryGATAN LIQUID NITROGEN°° eV K K K mrad e/Å2
Processing
Software:SIMPLE
Resolution by author:8 Å
Resolution method:0.5 FSC
Unit cell:
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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