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Title:The reconstructed F120 amyloid fibril represents the structure of a selected subpopulation from the Abeta(1-40) fibril sample with a mean crossover distance of 120 nm. The F140 subpopulation with a mean crossover distance of 140 nm had been studied and deposited previously (EMDB accession no. 5008).
Authors:Sachse C, Grigorieff N, Faendrich M
Sample:Human Abeta (1-40)
Aggregation state:Helical (10.1 angstroms resolution)
Red flagLatest update:2011-01-10
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Sample
Sample name: Human Abeta (1-40)
Oligomeric state: helical
Theoretical molecular weight of the sample: 0.004330
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1proteinAbetafalseHomo sapiens
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
8.71 mg/mL50 mM BorateBlot for 7 seconds before plungingQuantifoil 400 mesh 1.3 micrometer holes
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%77.2 Kcustom-built plunging apparatusBlot for 7 seconds before plunging msin coldroom at 277 K.
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F30300 kVFLOOD BEAMBRIGHT FIELD2 mm1900 nm3500 nm5900058333FIELD EMISSION GUNKodak SO163 film mmobjective lens astigmatism was corrected at 200,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN°° eV93 K K K mrad35 e/Å209-Nov-2005
Processing
Software:SPIDER
CTF correction:Each particle CTFTILT
Resolution by author:10.1 Å
Resolution method:10.1
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
627 μm/pixellinkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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