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Title:RNA-releasing poliovirus intermediates
Authors:H.C.Levy,M.Bostina,D.J.Filman,J.M.Hogle
Sample:80S poliovirus
Aggregation state:Icosahedral (10 angstroms resolution)
Red flagLatest update:2011-09-27
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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Sample
Sample name: 80S poliovirus
Oligomeric state: 60 promoters arranged as a icosahedron
Theoretical molecular weight of the sample: 8.3
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1viruspoliovirus 1 mahoney8.3poliovirus 1 mahoney00.052.0.01.001native virus is converted by heat-treatment to 80S
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.40.2 mg/mL20mM Tris, 50mM NaCl, 2 mM CaCl2not stained200 mesh copper grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGERblot for 3 secs msVitrification instrument: home made plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F30200 kVOTHEROTHER mm900 nm3000 nm59000FIELD EMISSION GUNKodak SO163 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV K K K mrad15 e/Å2
Processing
Software:PFT2, EM3DR2
CTF correction:each micrograph
Resolution by author:10 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1387 μm/pixellinkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1pov Rigid BodyINSOUTRECIPROCAL
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