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Title:Clathrin D6 coat
Authors:A.Fotin,Y.Cheng,P.Sliz,N.Grigorieff,S.C.Harrison,T.Kirchhausen,T.Walz
Sample:Clathrin coats
Aggregation state:Single particle (7.9 angstroms resolution)
Red flagLatest update:2010-05-05
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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Sample
Sample name: Clathrin coats
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinclathrin coatD6 assemblefalseBos taurus
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
6.51 mg/mL25mM MES pH 6.5, 2mM DTTholey carbon grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%93 KFEI VITROBOT msVitrification instrument: FEI Vitrobot
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2 mm2000 nm5000 nm5000051159FIELD EMISSION GUNKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN°° eV93 K K K mrad e/Å2
Processing
Software:Frealign
CTF correction:CTF correction of each particle.
Resolution by author:7.9 Å
Resolution method:FSC at 0.143 cut-off
Processing details:This is an n.c.s averaged map from raw reconstruction
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
2257 μm/pixel1.48linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1N4C 1BPO 1B89 Rigid BodyDENSITY CORRELATIONOREALvarious segments of clathrin were separately fitted by manual docking using program O, and fitting was improved by MAVE
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