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Title:Molecular Structure of the Native HIV-1 gp120 trimer bound to CD4 and 17b: Spike region
Authors:A.Bartesaghi,M.Borgnia,J.Liu,G.Sapiro,S.Subramaniam
Sample:HIV-1 BaL virus
Aggregation state:Individual structure (20 angstroms resolution)
Red flagLatest update:2011-08-02
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bullet_white.gif  Summary
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Sample
Sample name: HIV-1 BaL virus
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusenvelope glycoproteinenvelope glycoprotein00.061.1.06.009
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.2 mg/mL0.01 M Tris, 0.1 M NaCl, 1 mM EDTAhome-made holey carbon
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100%77 KFEI VITROBOTBlot and plunge msVitrification instrument: Vitrobot
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI POLARA 300200 kVFLOOD BEAMBRIGHT FIELD2.2 mm2000 nm4000 nm34000FIELD EMISSION GUNCCD mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
CartridgeGATAN LIQUID NITROGEN-70°70°Gatan0-20 eV77 K K K mrad80 e/Å2
Processing
Software:IMOD and in-house procedures
Resolution by author:20 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1GC1 correlation coefficientChimeraREALAutomated fitting procedures
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