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Title:Paired beta-sheet structure of an Abeta(1-40) amyloid fibril revealed by electron microscopy
Authors:C.Sachse,M.Faendrich,N.Grigorieff
Sample:human Abeta(1-40)
Aggregation state:Helical (8.8 angstroms resolution)
Red flagLatest update:2009-10-14
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bullet_white.gif  Summary
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Sample
Sample name: human Abeta(1-40)
Theoretical molecular weight of the sample: 0.004330
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinAbetafalseIPR013803
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.81 mg/mL50 mM BorateQuantifoil R1.2/1.3 Cu 400 mesh grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%77.2 KNONEBlot for 7 seconds before plunging msVitrification carried out in cold room at 277 K.
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F30300 kVFLOOD BEAMBRIGHT FIELD2.0 mm1900 nm3500 nm5900058333FIELD EMISSION GUNKodak SO163 film mmobjective lens astigmatism was corrected at 200,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN0°0° eV93 K K K mrad35 e/Å209-Nov-2005
Processing
Software:SPIDER
CTF correction:Each particle CTFTILT
Resolution by author:8.8 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
627 μm/pixel8linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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