top_cornerHomeEMD-1699  Contact us 
Image unavailable
Title:Structure of Lactococcal Phage p2 Baseplate and its Mechanism of Activation
Authors:G.Sciara,C.Bebeacua,P.Bron,D.Tremblay,M.Ortiz-Lombardia,J.Lichiere,M.van Heel,V.Campanacci,S.Moineau,C.Cambillau
Sample:P2 baseplate wild-type
Aggregation state:Single particle (22 angstroms resolution)
Red flagLatest update:2010-06-01
inner_corner
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: P2 baseplate wild-type
Oligomeric state: Homohexamer
Theoretical molecular weight of the sample: 1
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinP2 baseplateHexamerfalseLactococcus phage p2
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mLSample was incubated on glow-discharged grid for approximately one minute. 2% uranyl acetate was applied onto the sample and left for about 30 seconds.
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG/UT200 kVSPOT SCANBRIGHT FIELD2.2 mm500 nm1500 nm38000FIELD EMISSION GUN mmcorrected at 200,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Room TemperatureOTHER0°0° eV K K K mrad10 e/Å2
Processing
Software:IMAGIC-5
CTF correction:CCD Images
Resolution by author:22 Å
Resolution method:FSC at half-bit cut-off
Processing details:Initial map calculated with class averages. Final map calculated after projection matching refinement.
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
10002.32 μm/pixellink
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
outer_corner