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Title:CryoEM Model of the Vesicular Stomatitis Virus
Authors:P.Ge,J.Tsao,T.J.Green,M.Luo,Z.H.Zhou
Sample:VSV (Indiana)
Aggregation state:Helical (10.6 angstroms resolution)
Red flagLatest update:2010-03-10
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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Sample
Sample name: VSV (Indiana)
Oligomeric state: Mature virion
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusVesicular stomatitis virusVesicular stomatitis virus01.062.0.01.008Horse is the primary host species of the virus but Human is also possible.
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.4 mg/mL100mM NaCl, 10nM Tris, 1mM EDTACryo sampleQuantifoil 3.5/1 200 mesh
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE50%80 KHOMEMADE PLUNGERmanual blot 1 second before plunging msVitrification instrument: manual plunger. Vitrification carried out in open room
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI POLARA 300300 kVSPOT SCANBRIGHT FIELD2 mm800 nm2500 nm5900098000FIELD EMISSION GUNTietz CCD mmObjective lens astigmatism was corrected at 250,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricSIDE ENTRY, EUCENTRIC°° eV80 K K K mrad20 e/Å205-JAN-2007
Processing
Software:EMAN
Resolution by author:10.6 Å
Resolution method:FSC at 0.5 cut-off
Processing details:Helicity determined and applied by IHRSR
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
2121.532 μm/pixel16link
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
2GIC Rigid BodyCross-correlationChimeraREAL
2W2R Rigid BodyCross-correlationChimeraREAL
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