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Title:The three-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopy
Authors:P.Luik,C.Chew,J.Aittoniemi,J.Chang,P.Wentworth,R.A.Dwek,P.C.Biggin,C.Venien-Bryan,N.Zitzmann
Sample:HCV p7 hexamer, JFH-1 strain, genotype 2a
Aggregation state:Single particle (16 angstroms resolution)
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: HCV p7 hexamer, JFH-1 strain, genotype 2a
Oligomeric state: Hexameric
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinp7falseHepatitis C virusp7 solubilzed with DHPC
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.0 mg/mL100 mM NaCl, 50 mM DHPC, 25 mM Hepes, pH 7.02% (w/v) phosphotungstic acid (PTA)
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM120T120 kVSPOT SCANBRIGHT FIELD mm nm nmLAB6Kodak SO 163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricPHILIPS ROTATION HOLDER°° eV K K K mrad e/Å2
Processing
Resolution by author:16 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixellinkNIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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