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Title:Specific arrangement of alpha-helical coiled coils in the core domain of the bacterial flagellar hook for the universal joint function
Authors:T.Fujii,T.Kato,K.Namba
Sample:Bacterial flagellar hook
Aggregation state:Helical (7.1 angstroms resolution)
Red flagLatest update:2009-11-17
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bullet_white.gif  Summary
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Sample
Sample name: Bacterial flagellar hook
Oligomeric state: Bacterial flagellar hook
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinBacterial flagellar hookfalseSalmonella enterica subsp. enterica serovar Typhimurium
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7 mg/mL20mM Tris-HCl, 100mM NaClQuantifoil holey carbon molybdenum grid R0.6/1.0
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
HELIUM90%4.6 KHOMEMADE PLUNGER msVitrification instrument: Vitorobot
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
OTHER200 kVFLOOD BEAMBRIGHT FIELD1.6 mm500 nm2000 nm5000089285FIELD EMISSION GUN16 megapixels slow-scan CCD camera, TemCam-F415MP mmObjective lens astigmatism was corrected at 100,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Top entry liquid helium-cooled cryo specimen holderJEOL°°0-10 eV50 K K K mrad20 e/Å220-Feb-2008
Processing
Software:SPIDER
CTF correction:Each particle
Resolution by author:7.1 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
20015 μm/pixel16link
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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