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Title:Structure of the Human Dicer-TRBP Complex by Electron Microscopy
Authors:Lau PW, Potter CS, Carragher B, Macrae IJ
Sample:Dicer-TRBP complex
Aggregation state:Single particle (20 angstroms resolution)
Red flagLatest update:2009-10-20
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: Dicer-TRBP complex
Oligomeric state: One human Dicer binds to one TRBP molecule
Theoretical molecular weight of the sample: 0.25
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1proteinHuman DicerMonomerfalseHomo sapiens
2proteinTAR RNA binding proteinMonomerfalseHomo sapiens
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.4 mg/mL150mM KCl, 20mM HEPESGrids with adsorbed protein floated on 2% w/v uranyl acetate for approximately 20 seconds.
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20120 kVFLOOD BEAMBRIGHT FIELD2.0 mm150 nm nm50000FIELD EMISSION GUNTietz F415 4 K x 4 K pixel CCD camera mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Single tilt room temperature grid holderSIDE ENTRY, EUCENTRIC°° eV K K K mrad20 e/Å231-MAY-2009
Processing
Software:EMAN, SPIDER
CTF correction:Whole image
Resolution by author:20 Å
Resolution method:FSC and Rmeasure at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
947 μm/pixellink
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
2ffl Rigid BodyChimeraREALManual docking
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