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Title:Structure of human erythrocyte anion exchanger 1 revealed by electron crystallography.
Authors:T.Yamaguchi,Y.Ikeda,Y.Abe,H.Kuma,D.Kang,N.Hamasaki,T.Hirai
Sample:Membrane domain of anion exchanger 1
Aggregation state:2D crystal (7.5 angstroms resolution)
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Sample
Sample name: Membrane domain of anion exchanger 1
Oligomeric state: Dimer
Theoretical molecular weight of the sample: 0.055
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinAnion Exchanger 10.055DimerfalseHomo sapiens
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
2.5 mg/mLMo grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NITROGEN%77 KHOMEMADE PLUNGERCarbon sandwich method msVitrification instrument: Manual. Excess buffer was removed by pipetting and blotting with filter paper, and then the grid was manually plunged into liquid nitrogen. These operations were carried out at 4 degrees celsius.
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 3000SFF300 kVFLOOD BEAMBRIGHT FIELD1.6 mm1000 nm2500 nm40000FIELD EMISSION GUNKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Top entryJEOL-60°60° eV4 K K K mrad e/Å2
Processing
Software:MRC
CTF correction:Each film
Resolution by author:7.5 Å
Resolution method:Phase residual
Unit cell:
abGamma
194.5 Å182.0 Å90°
Plane/space group:P 2 21 21
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
317 μm/pixel12linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1OTS Rigid BodyFree RCNSRECIPROCAL
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