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Title:A dimeric structure for archaeal methylation-guide small ribonucleoproteins
Authors:F.Bleichert,K.T.Gagnon,B.A.Brown II,E.S.Maxwell,A.E.Leschziner,V.M.Unger,S.J.Baserga
Sample:Methanocaldococcus jannaschii methylation-guide sRNP
Aggregation state:Single particle (27 angstroms resolution)
Red flagLatest update:2009-10-20
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: Methanocaldococcus jannaschii methylation-guide sRNP
Theoretical molecular weight of the sample: 0.366
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinRibonucleoproteintrueMethanocaldococcus jannaschii
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.0 mg/mL20mM Hepes, 500mM NaCl, 1.5mM MgCl2Sandwiched, 2% uranyl formate
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI 12120 kVFLOOD BEAMBRIGHT FIELD mm nm nm52000LAB6Kodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricOTHER0°50° eV K K K mrad20 e/Å2
Processing
Software:SPIDER
Resolution by author:27 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixellinkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
2nnw Situs, ChimeraREAL
1xbi Situs, ChimeraREAL
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