top_cornerHomeEMD-1619  Contact us 
Image unavailable
Title:Three-dimensional Structure of Tropism-Switching Bordetella Bacteriophage
Authors:W.Dai,A.Hodes,W.H.Hui,M.Gingery,J.F.Miller,Z.H.Zhou
Sample:Bvg plus tropic phage-1 (BPP-1) bacteriophage
Aggregation state:Single particle (40 angstroms resolution)
Red flagLatest update:2011-09-09
inner_corner
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: Bvg plus tropic phage-1 (BPP-1) bacteriophage
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusBPP-1 bacteriophageBPP-1 bacteriophage
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL200 mesh grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KNONEBlot for 1 second before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20300 kVFLOOD BEAMBRIGHT FIELD2 mm4000 nm6000 nm2300038200FIELD EMISSION GUNCCD mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN-70°70° eV K K K mrad1 e/Å2
Processing
Software:ProTomo
Resolution by author:40 Å
Processing details:Final map was averaged from 60 particles
Unit cell:
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
outer_corner