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Title:The structures of bacteriophages K1E and K1-5 explain processive degradation of polysaccharide capsules and evolution of new host specificities.
Authors:Petr G Leiman, Anthony J Battisti, Valorie D Bowman, Katharina Stummeyer, Martina Muehlenhoff, Rita Gerardy-Schahn, Dean Scholl, Ian J Molineu
Sample:Sixfold averaged tail of bacteriophage K1E
Aggregation state:Single particle (16.4 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: Sixfold averaged tail of bacteriophage K1E
Theoretical molecular weight of the sample: 8
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusbacteriophage K1E tail8bacteriophage K1E tailsixfold averaged
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.51 mg/mL50 mM Tris HCl pH 7.5, 100 mM NaCl, 8 mM MgSO4nonequantifoil grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE80%300 KFEI VITROBOT msVitrification instrument: Vitrobot
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM300FEG/T300 kVSPOT SCANBRIGHT FIELD2.0 mm700 nm3300 nm4500047000FIELD EMISSION GUNKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN°° eV100 K K K mrad25 e/Å22005-05-10
Processing
Software:Spider
CTF correction:Each particle
Resolution by author:16.4 Å
Resolution method:FSC at 0.4 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1227 μm/pixel12linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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