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Title:Electron cryotomography reveals the portal in the herpesvirus capsid.
Authors:J.T.Chang, M.F.Schmid, F.J.Rixon, W.Chiu
Sample:Herpes Simplex Virus 1
Aggregation state:Individual structure
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: Herpes Simplex Virus 1
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusHerpes SimplexHerpes Simplex
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.2 mg/mL170mM NaCl, 3.4mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, 6.8mM CaCl2, 4.9mM MgCl2200 mesh copper grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100%293 KFEI VITROBOT msVitrification instrument: Vitrobot
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 2010F200 kVFLOOD BEAMBRIGHT FIELD2 mm3000 nm3000 nm2000027680FIELD EMISSION GUNGatan mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen cooledGATAN LIQUID NITROGEN70°70° eV93 K93 K93 K mrad50 e/Å2Tilted from -70 to +70 in 2 degree increments using Mr T software (unpublished).
Processing
Software:IMOD
Processing details:5-fold-averaged
Unit cell:
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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