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Title: Centrosome polarization delivers secretory granules to the immunological synapse. Authors: Stinchcombe JC, Majorovits E, Bossi G, Fuller S, Griffiths GM Aggregation State: individualStructure, (resolution 50 Angstroms)   Summary   Visualisation   Sample  Experiment   Processing   Downloads Experiment Sample Preparation: ph: Sample Conc.: Details: Staining: Sample Support Details: RPMI medium CTLs were labelled overnight with 1-2mg/ml HRP to load the lytic granules. After 30 min. conjugation to P815 target cells with anti-CD3 antibodies the sample was fixed and processed for DAB-cytochemistry and post-fixed with reduced osmium. The serial section was stained with lead citrate for 8 min. Vitrification: Cryogen Name: Humidity: Temp.: Instr.: Method: Time Resolved: Details: NONE Imaging Conditions: Microscope: Voltage: Illumination Mode: Imaging Mode: Cs: Defocus Min: Defocus Max: Nominal Mag: Calibrated Mag: Electron Source: Detector: Detector distance: Astigmatism: FEI TECNAI F30 300 FLOOD BEAM BRIGHT FIELD 2.2 4800 5200 5600 6200 FIELD EMISSION GUN GATAN 2kx2k CCD camera Specimen Holder: Holder Model: Tilt Min: Tilt Max: Energy Filter: Energy Window: Temp.: Temp. min: Temp. max: Beam Tilt: Electron Dose: Other Details: Date: flip-flop holder OTHER -68.0 68.0 GIF 0-20 double tilt series 2005-11-08