top_cornerHomeEMD-1211  Contact us 
Image unavailable
Title:Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunit.
Authors:T. Schafer, B. Maco, E. Petfalski, D. Tollervey, B. Bottcher, U. Aebi, E. Hurt
Sample:pre-40S ribosome
Aggregation state:Single particle (34 angstroms resolution)
Red flagLatest update:2011-05-26
inner_corner
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: pre-40S ribosome
Oligomeric state: monomer
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1ribosome-eukaryotepre-40S ribosomeLtv1 Tsr1 Enp1 Nob1 Hrr25 Rio2 Dim1 Dim2 Ltv1 used as bait in TAP-purification
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.5 mg/mL50mM Tris-HCl, 100mM NaCl, 10mM MgCl2, 0.5mM DTT400 mesh copper rhodium grids (Maxtaform)
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE90%295 KHOMEMADE PLUNGERBlot for 15 s with Whatman No 1 msVitrification instrument: Plunger with environmental chamber
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG200 kVFLOOD BEAMBRIGHT FIELD2.0 mm2200 nm4400 nm50000FIELD EMISSION GUNCCD mmbjective lens astigmatism was corrected at

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN0°0° eV94 K K K mrad e/Å2
Processing
Software:IMAGIC 5
CTF correction:Each Particle
Resolution by author:34 Å
Resolution method:FSC at 0.5 cut-off
Processing details:reconstructions were calculated from half of the class averages. Euler angles of class averages were determined by projection matching
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
6914 μm/pixel12link
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
outer_corner