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Title:Interactions of the release factor RF1 with the ribosome as revealed by cryo-EM.
Authors:U. Rawat, H.Gao, A. Zavialov, R. Gursky, M. Ehrenberg, J. Frank
Sample:Release complex bound with RF1-WT
Aggregation state:Single particle (12.8 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: Release complex bound with RF1-WT
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1ribosome-prokaryote30S
2ribosome-prokaryote50S
3nucleic-acidP-site tRNAfalse
4nucleic-acidE-site tRNAfalse
5nucleic-acidmRNAtrue
6proteinRF1falseunidentified
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE90%93 KHOMEMADE PLUNGERBlot for 2 seconds before plunging msVitrification instrument: two sided blotting plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2 mm2000 nm4000 nm5000049696FIELD EMISSION GUNKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Cryo stageGATAN LIQUID NITROGEN0°0° eV93 K K K0 mrad20 e/Å22002-12-01
Processing
Software:SPIDER package
CTF correction:defocus groups
Resolution by author:12.8 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
5614 μm/pixel1.212linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
2FVO Rigid Bodycorrelation coefficientOREALmanual fitting in O
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