top_cornerHomeEMD-1178  Contact us 
Image unavailable
Title:Structure of the dodecahedral penton particle from human adenovirus type 3.
Authors:Fuschiotti P, Schoehn G, Fender P, Fabry CM, Hewat EA, Chroboczek J, Ruigrok RW, Conway JF
Sample:adenovirus 3 penton base dodecahedron
Aggregation state:Icosahedral (9.3 angstroms resolution)
Red flagLatest update:2011-05-26
inner_corner
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: adenovirus 3 penton base dodecahedron
Oligomeric state: 12 pentamers
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1proteinAd3 penton base0.060dodecamer of pentamertruehuman adenovirus 3
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
6.61 mg/mL25 mM phosphate buffer at pH 6.6Quantifoil R2 1 grids (Quantifoil Micro Tools GmbH, Germany) were loaded with 4 ul of sample at 1 mg ml, blotted and rapidly frozen in liquid ethane within a liquid nitrogen bath using a Zeiss cryoplungerQuantifoil R2/1 grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%100 KZeiss cryoplunger ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 2010F200 kVOTHERBRIGHT FIELD1.4 mm1000 nm2500 nm5000051100FIELD EMISSION GUNKodak SO163 film mmobjective lens astigmatism was corrected at 100,000

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricOTHER°° eV100 K K K mrad e/Å2
Processing
Software:PFT EM3DR PFT2 EM3DR2
CTF correction:each negative or each focal pair
Resolution by author:9.3 Å
Resolution method:FSC 0.33
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
147 μm/pixellinkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
outer_corner