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Title: The structure of the poliovirus 135S cell entry intermediate at 10-angstrom resolution reveals the location of an externalized polypeptide that binds to membranes.
Authors: Bubeck D, Filman DJ, Cheng N, Steven AC, Hogle JM, Belnap DM
Aggregation State: icosahedral, (resolution 11 Angstroms)
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Experiment
Sample Preparation:
ph:Sample Conc.:Details:Staining:Sample Support Details:
7.420 mM HEPES, 2 mM CaCl2
Vitrification:
Cryogen Name:Humidity:Temp.:Instr.:Method:Time Resolved:Details:
ETHANEPlunge frozen in liquid ethane.Vitrification occurred in ambient atmosphere.
Imaging Conditions:
Microscope:Voltage:Illumination Mode:Imaging Mode:Cs:Defocus Min:Defocus Max:Nominal Mag:Calibrated Mag:Electron Source:Detector:Detector distance:Astigmatism:
FEI TECNAI 20200FLOOD BEAMBRIGHT FIELD50000FIELD EMISSION GUN
Specimen Holder:Holder Model:Tilt Min:Tilt Max:Energy Filter:Energy Window:Temp.:Temp. min:Temp. max:Beam Tilt:Electron Dose:Other Details:Date:
Side entry, liquid nitrogen-cooled, cryo specimen holderGATAN LIQUID NITROGEN0010
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