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Title:Structure of the hepatitis C virus IRES bound to the human 80S ribosome: remodeling of the HCV IRES.
Authors:D. Boehringer, R. Thermann, A.
Sample:IRES-80S ribosome complex
Aggregation state:Single particle (15 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: IRES-80S ribosome complex
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1cellular-componentribosomefalseH. sapiensH. sapiens
2nucleic-acidHCV IREStrue
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
8.1 mg/mL20 mM Tris.HCl pH 8.1, 145 mM KCl, 1 mM CaCl2, 5 mM MgCl2, 0.2 mM DTT, 5 mM tobramycinnative cryocopper grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG200 kVSPOT SCANBRIGHT FIELD mm2000 nm4000 nm50000FIELD EMISSION GUNKodak SO163 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricOTHER°° eV K K K mrad e/Å2
Processing
Software:imagic 5
Resolution by author:15 Å
Resolution method:FSC 3 sigma
Unit cell:
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
2AGN 
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