top_cornerHomeEMD-1136  Contact us 
Image unavailable
Title:The structure of the poliovirus 135S cell entry intermediate at 10-angstrom resolution reveals the location of an externalized polypeptide that binds to membranes.
Authors:Bubeck D, Filman DJ, Cheng N, Steven AC, Hogle JM, Belnap DM
Sample:Poliovirus 135S particle
Aggregation state:Icosahedral (8.7 angstroms resolution)
Red flagLatest update:2011-05-26
inner_corner
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: Poliovirus 135S particle
Oligomeric state: icosahedrally ordered capsid, 60 copies of VP1, VP2, VP3
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1virusHuman poliovirus 1, Mahoney strain.Complete virus identifier:ICTVdb 00.052.0.01.007Human poliovirus 1, Mahoney strain.Complete virus identifier:ICTVdb 00.052.0.01.00700.052.0.01.007135S particle
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.5 mg/mL20 mM HEPES, 2 mM CaCl2, 0.07 M NaCl
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KBlotted manually before plunging msVitrification carried out in ambient atmosphere. Ethane cooled by liquid nitrogen.
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG120 kVFLOOD BEAMBRIGHT FIELD2.0 mm nm nm3800038500FIELD EMISSION GUNKodak SO-163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry, liquid nitrogen-cooled, cryo specimen holderGATAN LIQUID NITROGEN0°0° eV K K K mrad10 e/Å2
Processing
Software:EM3DR2
CTF correction:CTF and decay correction of each particle image
Resolution by author:8.7 Å
Resolution method:FSC at 0.3 cut-off
Processing details:Reconstructed computed from focal pairs. Pairs not summed for reconstruction calculaton.
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
127.0 μm/pixel8linkSix defocal pairs were scanned.ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
rigid bodyRECIPROCALsee paper for details of the model fitting
outer_corner