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Title: The structure of the poliovirus 135S cell entry intermediate at 10-angstrom resolution reveals the location of an externalized polypeptide that binds to membranes.
Authors: Bubeck D, Filman DJ, Cheng N, Steven AC, Hogle JM, Belnap DM
Aggregation State: icosahedral, (resolution 8.7 Angstroms)
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Processing
Software:EM3DR2
CTF Correction:CTF and decay correction of each particle image
Resolution By Author:8.7
Resolution Method:FSC at 0.3 cut-off
Processing details:Reconstructed computed from focal pairs. Pairs not summed for reconstruction calculaton.
Unit Cell:
Scanned images:
Digital Images:Sampling Size:OD Range:Quant. Bit Number:Other details:Scanner:
127.08Six defocal pairs were scanned.ZEISS SCAI
Fitting:
Prot
-ocol:
Target Crit.:Soft
-ware:
B value:Ref. Space:PDB ID:PDB chain ID:Details:
rigid bodyRECIPROCALsee paper for details of the model fitting
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