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Title:Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly.
Authors:Wang HW, Nogales E
Sample:GDP-tubulin
Aggregation state:Helical (12 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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Sample
Sample name: GDP-tubulin
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1proteintubulin0.11dimerfalse
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
6.82 mg/mL80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, 1 mM GDP, 30 mM MnCl2400 mesh Quantifoil grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100%90 KVitrobotBlot for 1.8 seconds before plunging, with Vitrobot offset set as -2 mm. ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG200 kVFLOOD BEAMBRIGHT FIELD2.2 mm600 nm2000 nm5000050000FIELD EMISSION GUNKodak SO163 film mmobjective lens astigmatism was corrected at 200K mag

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV90 K K K mrad15 e/Å2
Processing
Software:MRC and home-made software
CTF correction:each image
Resolution by author:12 Å
Resolution method:layer line with signal-to-noise ratio higher than 2.0
Processing details:Final maps were calculated from 19 tube images
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
20012.7 μm/pixel14linkOTHER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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