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EMDB Entry EMD-1129
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Title
:
Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly.
Authors
:
Wang HW, Nogales E
Source
:
GDP-tubulin
Aggregation State
:
helical, (resolution 12 Angstroms)
Summary
Visualisation
Sample
Experiment
Processing
Downloads
Experiment
Sample Preparation:
ph:
Sample Conc.:
Details:
Staining:
Sample Support Details:
6.8
2.0
80 mM PIPES, 1 mM MgCl2, 1mM GDP, 30 mM MnCl2
400 Quantifoil
Vitrification:
Cryogen Name:
Humidity:
Temp.:
Instr.:
Method:
Time Resolved:
Details:
ETHANE
100
90
Vitrobot
Blot for 1.8 seconds before plunging with Vitrobot offset as -2 mm
Imaging Conditions:
Microscope:
Voltage:
Illumination Mode:
Imaging Mode:
Cs:
Defocus Min:
Defocus Max:
Nominal Mag:
Calibrated Mag:
Electron Source:
Detector:
Detector distance:
Astigmatism:
FEI/PHILIPS CM200 FEG
200
FLOOD BEAM
BRIGHT FIELD
2.2
600
2000
50000
50000
FIELD EMISSION GUN
Kodak SO163 film
objective lens astigmatism was corrected at 200K mag
Specimen Holder:
Holder Model:
Tilt Min:
Tilt Max:
Energy Filter:
Energy Window:
Temp.:
Temp. min:
Temp. max:
Beam Tilt:
Electron Dose:
Other Details:
Date:
Eucentric
GATAN LIQUID NITROGEN
0
0
90
15
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