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Title:Conservation of the capsid structure in tailed dsDNA bacteriophages: the pseudoatomic structure of phi29.
Authors:Morais MC, Choi KH, Koti JS, Chipman PR, Anderson DL, Rossmann MG
Sample:fibered isometric phi29 particle
Aggregation state:Icosahedral (8.7 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: fibered isometric phi29 particle
Oligomeric state: T3 icosahedron
Theoretical molecular weight of the sample: 14.2
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1virusphi2914.2phi2902.054.0.02.001
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.8 mg/mL25 mM Tris 5 mM MgCl2 50 mM NaCl 2 mM sodium azideholey carbon
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%113 K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG200 kVFLOOD BEAMBRIGHT FIELD2.0 mm1091 nm3903 nm38000FIELD EMISSION GUNKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN0.0°0.0° eV K K K mrad20 e/Å2
Processing
Software:PFT P3DR POR
CTF correction:CTF correction, phases and amplitudes, for each micrograph
Resolution by author:8.7 Å
Resolution method:FSC at 0.5
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
291.84 μm/pixel8linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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