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Title:A quasi-atomic model of human adenovirus type 5 capsid.
Authors:Fabry CM, Rosa-Calatrava M, Conway JF, Zubieta C, Cusack S, Ruigrok RW, Schoehn G
Sample:Human adenovirus type 5 pIX deletion mutant
Aggregation state:Icosahedral (22 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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Sample
Sample name: Human adenovirus type 5 pIX deletion mutant
Theoretical molecular weight of the sample: 150
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1virusadenovirus150adenovirus
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.50.8 mg/mL150 mM NaCl 20 mM Tris-HCl pH 7.5Cryo EMQuantifoil grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%90 KZeiss plungerManual blot for 1-2 seconds before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200T200 kVOTHERBRIGHT FIELD2 mm100 nm250 nm2750028050LAB6Kodak SO163 film mmobjective lens astigmatism was corrected at 100,000

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV90 K90 K90 K mrad10 e/Å22003-11-01
Processing
Software:PFT EM3DR
CTF correction:CTFMIX
Resolution by author:22 Å
Resolution method:FSC at 0.3 cut-off
Processing details:Final maps were calculated from 660 single images
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
107 μm/pixel1.28linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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