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Title:Global conformational rearrangements during the activation of the GDP/GTP exchange factor Vav3.
Authors:O. Llorca, E. Arias-Palomo, J.L. Zugaza, X.R. Bustelo
Sample:Phosphorylated and active form of Vav3
Aggregation state:Single particle (22.0 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: Phosphorylated and active form of Vav3
Oligomeric state: monomer
Theoretical molecular weight of the sample: 0.098
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinphosphorylated vav30.098MonomertrueHomo sapiensGO:0007264
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.50.05 mg/mL50 mM Tris-Hcl, 100 mM NaClGlow-dischrged carbon-coated grids and negatively stained with 1% w/v uranyl acetate400 mesh copper-rhodium grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
OTHER100 kVFLOOD BEAMBRIGHT FIELD2.9 mm nm nm50000TUNGSTEN HAIRPINKodaK 4489 film mmcorrection with CCD camera

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricOTHER0°0° eV293 K K K mrad e/Å2Electron microscope:JEOL JEM-1230, 120kV. Specimen holder: JEOL type M:207EM-11020.2003-11-14
Processing
Software:EMAN
Resolution by author:22.0 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
10 μm/pixel16linkDigitiser used: Minolta Dimage Scan Multi ProOTHER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1H67 1F5X 1AWE 1TBO 1GRI Rigid BodyCorrelation coeficientsSITUSThe domains were fitted computationally using the SITUS software
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