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Title:Cryo-EM visualization of a viral internal ribosome entry site bound to human ribosomes: the IRES functions as an RNA-based translation factor.
Authors:Spahn CM, Jan E, Mulder A, Grassucci RA, Sarnow P, Frank J
Sample:Human ribosome 40S subunit bound with CrPV IRES
Aggregation state:Single particle (20.3 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: Human ribosome 40S subunit bound with CrPV IRES
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1ribosome-eukaryoteHuman ribosome 40s subunit
2proteinCrPV IRESfalsen/a
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm nm nm5000052000FIELD EMISSION GUNKodak SO163 film mmobjective lens astigmatism was corrected at 100,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
cryo transferOTHER eV93 K K K mrad15 e/Å2Cryo-transfer stage made by Oxford instruments, UK.
Processing
Software:SPIDER
CTF correction:defocus group volumes
Resolution by author:20.3 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
14 μm/pixellinkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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