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Title:Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host.
Authors:Leiman PG, Chipman PR, Kostyuchenko VA, Mesyanzhinov VV, Rossmann MG
Sample:T4 phages treated with 3 M urea
Aggregation state:Single particle (18 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: T4 phages treated with 3 M urea
Theoretical molecular weight of the sample: 220
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1virusbacteriophage T4220bacteriophage T402.043.0.01.005treated with 3 M urea
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.55 mg/mLH2O200 mesh cupper grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%100 K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM300FEG/T300 kVSPOT SCANBRIGHT FIELD mm500 nm3400 nm4500047000FIELD EMISSION GUNKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN0°0°FEI eV100 K K K mrad20 e/Å22002-01-06
Processing
Software:Spider
CTF correction:Each particle
Resolution by author:18 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1004.51104 μm/pixel8linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
The components were separately fitted using Situs v.2.0.
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