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Title:Structure of the 80S ribosome from Saccharomyces cerevisiae--tRNA-ribosome and subunit-subunit interactions.
Authors:Spahn CM, Beckmann R, Eswar N, Penczek PA, Sali A, Blobel G, Frank J
Sample:the 80S Ribosome from Saccharomyces cerevisiae
Aggregation state:Single particle (15.4 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: the 80S Ribosome from Saccharomyces cerevisiae
Theoretical molecular weight of the sample: 3.2
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1ribosome-eukaryote80S ribosome
2ligandtRNAfalse
3ligandSec61false
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm1700 nm6000 nm5000052000FIELD EMISSION GUNKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
cryo-transferGATAN LIQUID NITROGEN°° eV93 K K K mrad e/Å2
Processing
Software:SPIDER
CTF correction:defocus group volumes
Resolution by author:15.4 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixel12linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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