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Title:Molecular architecture of the prolate head of bacteriophage T4.
Authors:Fokine A, Chipman PR, Leiman PG, Mesyanzhinov VV, Rao VB, Rossmann MG
Sample:bacteriophage T4 prolate head
Aggregation state:Single particle (22.5 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: bacteriophage T4 prolate head
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Mutant Organism GO identifier InterPro identifier Virus identifier Details
1virusbacteriophage T4bacteriophage T443.0.1.0.001
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
6.2 mg/mLDistilled water. The concentration of the virus particles was 10E11 particles pre ml.quantifoil copper grid 2um holes
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%90 Kin-house, gravity driven plunger3.5ul of sample hand blotted approx. 1sec ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM300FEG/T300 kVFLOOD BEAMBRIGHT FIELD2.0 mm1100 nm2700 nm4500047000FIELD EMISSION GUNKodak SO-163 mmlive fft at 190,000x

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
naGATAN LIQUID NITROGEN°° eV97 K K K mrad29 e/Å2
Processing
Software:SPIDER
CTF correction:Each particle
Resolution by author:22.5 Å
Resolution method:FCS at 0.3 cut-off
Processing details:The reconstruction was performed by imposing 5-fold symmetry averaging. The 5-fold axis is along Z.
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
557 μm/pixel8linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
Nothing to fit
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