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| Title: | Three-dimensional structures of translating ribosomes by Cryo-EM. |
| Authors: | Gilbert RJ, Fucini P, Connell S, Fuller SD, Nierhaus KH, Robinson CV, Dobson CM, Stuart DI |
| Sample: | E. coli ribosome translating 2 Ig domains |
| Aggregation state: | Single particle (13.2 angstroms resolution) |
 Latest update: | 2011-05-26 |
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| Processing |
| Software: | SPIDER, IMAGIC, GAP, CNS, XPLOR |
| CTF correction: | Each negative dataset |
| Resolution by author: | 13.2 Å
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| Resolution method: | FSC at 0.5 cut-off |
| Processing details: | Final maps were calculated from 4434 images from
a total of 8898 from 14 individual datasets,
with scaling in reciprocal space to
crystallographic ribosome structures and correction
for map anisotropy by B-factor weighting
of amplitudes in XPLOR with respect to a
similarly-treated control inactive ribosome.
Selection of particles for inclusion in the
final maps was by correlation coefficient with
respect to the alignment model, designed to maximise
nascent chain occupancy in the selected images. |
| Unit cell: |
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| Scanned images: |
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| Fitting: |
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